Weak-affinity chromatography

  title={Weak-affinity chromatography},
  author={David Zopf and Sten Ohlson},
Weak-affinity chromatography is a new method using readily reversible biospecific recognition as the basis for chromatographic separations. 

Use of weak monoclonal antibodies for affinity chromatography

The potential of weak affinity biological interactions as a basis for chromatographic separation clearly demonstrates the potential of traditional affinity chromatography.

Evaluation of Rate Constants from Protein-Ligand Interactions with Weak Affinity Chromatography

The paradigm of drug discovery have been to find the strongest possible binder to the target by high-throughput screening (HTS) but high affinity interactions are related to low kinetic off rates a

Molecular Interactions in Bioseparations

General Introduction: Affinity Chromatography with Biological Ligands: Applications of Bacterial immunoglobulinBinding Proteins to the Purification of Immunoglobulins.

New methods for separation and recovery of biomolecules.


A simple model is presented that describes the competitive adsorption of two components in a weak affinity adsorption system. Unlike previous models, the approach used here allows description of

Comparison of Purification Efficiencies of Different Affinity Separation Techniques

An affinity separation method for the isolation of recombinantly produced benzoylformiate decarboxylase (BFD) is presented and it was shown that the disadvantages prevail and boronic acid resins are not suitable for protein purification from complex matrices.

Prediction of affinity and kinetics in biomolecular interactions by affinity chromatography.

This work has demonstrated the usefulness of the simulation approach for determination of affinity and kinetics in affinity chromatography, typically difficult to obtain for many weakly interacting molecular species.

Perspectives on immobilized proteins.




Paralog chromatography.

The construction of a representative set of paratope analogs, or "paralogs," which can be conjugated to a chromatographic sorbent to combine desirable characteristics of traditional high-performance liquid chromatography columns with the specificity of a moderate affinity antibody are described.

Novel approach to affinity chromatography using "weak" monoclonal antibodies.

Binding studies on substrate- and enantio-selective molecularly imprinted polymers

A molecularly imprinted polymer was prepared using tert-butyloxycarbonyl-L-phenylalanine as the print molecule and methacrylic acid as the functional monomer. The bulk polymer obtained was ground,

Computer simulation of weak affinity chromatography

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products. Four major

Radioimmunoassay of a glucose-containing tetrasaccharide using a monoclonal antibody.