Waxy Chlamydomonas reinhardtii: monocellular algal mutants defective in amylose biosynthesis and granule-bound starch synthase activity accumulate a structurally modified amylopectin.

@article{Delrue1992WaxyCR,
  title={Waxy Chlamydomonas reinhardtii: monocellular algal mutants defective in amylose biosynthesis and granule-bound starch synthase activity accumulate a structurally modified amylopectin.},
  author={Brigitte Delrue and T Juan Andr{\'e}s Fontaine and Françoise H Routier and Andr{\'e} Decq and Jean Michel Wieruszeski and N Van den Koornhuyse and M. L. Maddelein and Bernard Fournet and Stuart R. Ball},
  journal={Journal of bacteriology},
  year={1992},
  volume={174 11},
  pages={
          3612-20
        }
}
Amylose-defective mutants were selected after UV mutagenesis of Chlamydomonas reinhardtii cells. Two recessive nuclear alleles of the ST-2 gene led to the disappearance not only of amylose but also of a fraction of the amylopectin. Granule-bound starch synthase activities were markedly reduced in strains carrying either st-2-1 or st-2-2, as is the case for amylose-deficient (waxy) endosperm mutants of higher plants. The main 76-kDa protein associated with the starch granule was either missing… CONTINUE READING

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