Wavelength-shifting molecular beacons

@article{Tyagi2000WavelengthshiftingMB,
  title={Wavelength-shifting molecular beacons},
  author={Sanjay Tyagi and Salvatore A. E. Marras and Fred Russell Kramer},
  journal={Nature Biotechnology},
  year={2000},
  volume={18},
  pages={1191-1196}
}
We describe wavelength-shifting molecular beacons, which are nucleic acid hybridization probes that fluoresce in a variety of different colors, yet are excited by a common monochromatic light source. The twin functions of absorption of energy from the excitation light and emission of that energy in the form of fluorescent light are assigned to two separate fluorophores in the same probe. These probes contain a harvester fluorophore that absorbs strongly in the wavelength range of the… CONTINUE READING

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