Wavelength dependence of the time course of fluorescence enhancement and photobleaching during irradiation of ethidium bromide-stained nuclei.

@article{Galassi2000WavelengthDO,
  title={Wavelength dependence of the time course of fluorescence enhancement and photobleaching during irradiation of ethidium bromide-stained nuclei.},
  author={Luigi Galassi},
  journal={European journal of histochemistry : EJH},
  year={2000},
  volume={44 4},
  pages={419-32}
}
  • Luigi Galassi
  • Published 2000 in European journal of histochemistry : EJH
The variation of fluorescence during irradiation of ethidium bromide-stained nuclei with the 458 nm argon laser line was measured at different wavelengths throughout the emission spectrum. When glycerol was used as a mountant, photoenhancement of fluorescence was observed at all wavelengths, but was greater at the shorter wavelengths. Fluorescence increased by almost one order of magnitude at 500 nm after 40 s of irradiation, compared with only about 10% at wavelengths longer than 600 nm after… CONTINUE READING

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