Water transport and estimated transmembrane potential during freezing of mouse oocytes

@article{Toner1990WaterTA,
  title={Water transport and estimated transmembrane potential during freezing of mouse oocytes},
  author={Mehmet Toner and Ernest G. Cravalho and David Randall Armant},
  journal={The Journal of Membrane Biology},
  year={1990},
  volume={115},
  pages={261-272}
}
The kinetics of water transport and the changes in transmembrane potential during freezing of mouse oocytes in isotonic phosphate buffered saline (PBS) were simulated using thermodynamic models. The permeability to water at 0°C,L pg , and the activation energy,E Lp , of metaphase II mouse oocytes from B6D2F1 mice were determined to be 0.044±0.008 μm/minatm and 13.3±2.5 kcal/mol during freezing at 2°C/min. The inactive cell volume was determined to be 0.214 with a correlation coefficient of 0… CONTINUE READING
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