Water channels in the plant plasma membrane cloned by immunoselection from a mammalian expression system.

@article{Kammerloher1994WaterCI,
  title={Water channels in the plant plasma membrane cloned by immunoselection from a mammalian expression system.},
  author={W. Kammerloher and Ute Fischer and G P Piechottka and Anthony R Sch{\"a}ffner},
  journal={The Plant journal : for cell and molecular biology},
  year={1994},
  volume={6 2},
  pages={187-99}
}
Expression in mammalian COS cells and an efficient microtiter-based strategy for immunoselection was used in a novel approach to identify genes encoding plant membrane proteins. COS cells were transfected with an Arabidopsis thaliana root cDNA library constructed in a bacterial mammalian shuttle vector and screened with an antiserum raised against purified deglycosylated integral plasma membrane proteins from A. thaliana roots. Antibodies directed against a prominent 27 kDa antigen led to the… CONTINUE READING
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