Water and urea permeability properties of Xenopus oocytes: expression of mRNA from toad urinary bladder.

@article{Zhang1991WaterAU,
  title={Water and urea permeability properties of Xenopus oocytes: expression of mRNA from toad urinary bladder.},
  author={R R Zhang and Alan S. Verkman},
  journal={The American journal of physiology},
  year={1991},
  volume={260 1 Pt 1},
  pages={C26-34}
}
The Xenopus oocyte was evaluated as an mRNA expression system for water and urea transporters. Osmotic water permeability (Pf) was measured from the time course of oocyte volume in response to osmotic gradients using a real-time imaging method. Diffusional water permeability (Pd) was measured by 3H2O efflux. In mature oocytes treated with collagenase to remove the follicular cell layer, Pf was 8.6 +/- 0.6 x 10(-4) (SD) cm/s (n = 32) at 25 degrees C and independent of the time after oocyte… CONTINUE READING