Water and nonelectrolyte permeability of isolated rat hepatocytes.

  title={Water and nonelectrolyte permeability of isolated rat hepatocytes.},
  author={Gianfranco D Alpini and Rita Anne Garrick and Mark J. Jones and Rui Nunes and Nicola Tavoloni},
  journal={The American journal of physiology},
  volume={251 6 Pt 1},
We have measured the diffusive permeability coefficients of isolated rat hepatocytes to 3H2O, [14C]urea, [14C]erythritol, [14C]mannitol, [3H]sucrose, and [3H]inulin, employing a technique previously developed for erythrocytes (Redwood et al., J. Gen. Physiol 64:706-729, 1974). Diffusion coefficients for the tracer molecules were measured in packed hepatocytes, supernatant fluid, and intracellular medium (lysed hepatocytes) and were calculated assuming one-dimensional semi-infinite diffusion… CONTINUE READING

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