Wastewater bacterial communities bring together broad-host range plasmids, integrons and a wide diversity of uncharacterized gene cassettes.

@article{Moura2010WastewaterBC,
  title={Wastewater bacterial communities bring together broad-host range plasmids, integrons and a wide diversity of uncharacterized gene cassettes.},
  author={Alexandra Moura and Isabel Henriques and Kornelia Smalla and Ant{\'o}nio C.M. Correia},
  journal={Research in microbiology},
  year={2010},
  volume={161 1},
  pages={58-66}
}
To investigate the mobile gene pool present in wastewater environments, total community DNA was obtained from two distinct raw effluents: urban and slaughterhouse wastewaters. Bacterial community structure was evaluated by DGGE analysis of 16S rRNA gene fragments. Detection of broad-host-range plasmid sequences and integrase encoding genes was carried out through PCR and Southern hybridization. Gene cassette libraries were obtained using primers targeting consensus sequences that flank gene… CONTINUE READING