WIF-B cells: an in vitro model for studies of hepatocyte polarity

  title={WIF-B cells: an in vitro model for studies of hepatocyte polarity},
  author={Gudrun Ihrke and Edward B. Neufeld and T Meads and Michael R. Shanks and Doris Cassio and Michel Laurent and Trina A Schroer and Richard E. Pagano and Ann L. Hubbard},
  journal={The Journal of Cell Biology},
  pages={1761 - 1775}
We have evaluated the utility of the hepatoma-derived hybrid cell line, WIF-B, for in vitro studies of polarized hepatocyte functions. The majority (> 70%) of cells in confluent culture formed closed spaces with adjacent cells. These bile canalicular-like spaces (BC) accumulated fluorescein, a property of bile canaliculi in vivo. By indirect immunofluorescence, six plasma membrane (PM) proteins showed polarized distributions similar to rat hepatocytes in situ. Four apical PM proteins were… CONTINUE READING


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