Volume-activated chloride channels contribute to cell-cycle-dependent regulation of HeLa cell migration.


The activation of volume-activated chloride Cl(-) channels has been implicated to play important roles in modulating cell cycle and cell migration. The aim of this study was to determine whether volume-activated Cl(-) channels are involved in cell-cycle-dependent regulation of cell migration in HeLa cells. Using techniques including cell-cycle synchronization, transwell migration assays and the patch-clamp technique, we demonstrate in this study that both the expression of volume-activated chloride current (I(Cl,vol)) and the potential of cell migration are cell-cycle-dependent; specifically, these events were high in G(0)/G(1) phase, low in S phase, and medium in G(2)/M phase. Moreover, the mean density of I(Cl,vol) was positively correlated to the rate of cell migration during cell-cycle progression. Additionally, endogenous suppression of I(Cl,vol) by transfecting cells with ClC-3 antisense oligonucleotides arrested cells in S phase and slowed cell migration. Collectively, our results suggest that volume-activated Cl(-) channels contribute to the cell-cycle-dependent regulation of cell migration.

DOI: 10.1016/j.bcp.2008.10.009
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@article{Mao2009VolumeactivatedCC, title={Volume-activated chloride channels contribute to cell-cycle-dependent regulation of HeLa cell migration.}, author={Jianwen Mao and Lixin Chen and Bin Xu and Lijing Wang and Weizhang Wang and Ming Li and Min Zheng and Hongzhi Li and Jiao Guo and Weidong Li and Tim J. C. Jacob and Liwei Wang}, journal={Biochemical pharmacology}, year={2009}, volume={77 2}, pages={159-68} }