Development of a hypoxanthine biosensor based on Immobilized xanthine oxidase chemically modified electrode
- Hu Shengshui, Liu chung-chiun
Nanomolar levels of the hypoxanthine in NaOH electrolyte cantaining copper (II) can be determined by anodic stripping voltammetry at a glassy carbon electrode. In the present article hypoxanthine-Cu+ is shown to be adsorbed on the electrode surface in the presence of an excess of copper(II). After accumulation period, hypoxanthine-Cu+ was stripped from the electrode surface and the anodic current coming near to the oxidation of Cu(I) to Cu(II) was measured. A linear calibration curve in the range of 5 nmol/L-1.5 mmol/L hypoxanthine, with a detection limit of 0.5 nmol/L hypoxanthine were obtained.