Voltage sensing by fluorescence resonance energy transfer in single cells.

@article{Gonzlez1995VoltageSB,
  title={Voltage sensing by fluorescence resonance energy transfer in single cells.},
  author={J E Gonz{\'a}lez and Roger Tsien},
  journal={Biophysical journal},
  year={1995},
  volume={69 4},
  pages={
          1272-80
        }
}
A new mechanism has been developed for achieving fast ratiometric voltage-sensitive fluorescence changes in single cells using fluorescence resonance energy transfer. The mechanism is based on hydrophobic fluorescent anions that rapidly redistribute from one face of the plasma membrane to the other according to the Nernst equation. A voltage-sensitive fluorescent readout is created by labeling the extracellular surface of the cell with a second fluorophore, here a fluorescently labeled lectin… CONTINUE READING

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