Vitrification of the inner perivitelline layer of chicken eggs for use in the sperm-egg interaction assay.


The sperm-egg interaction assay is a good predictor of the fertilizing potential of rooster semen; the ability of chicken sperm to interact with the egg can be assessed by counting the number of holes in the inner perivitelline layer (IPVL) of a freshly laid egg. Although isolated IPVL can be stored for up to 24h, preservation of IPVL for prolonged… (More)
DOI: 10.1016/j.theriogenology.2009.02.009