Vitrification of mouse oocytes using a nylon loop.

  title={Vitrification of mouse oocytes using a nylon loop.},
  author={Michelle T Lane and David K. Gardner},
  journal={Molecular reproduction and development},
  volume={58 3},
Cryopreservation of mouse oocytes was improved by the use of ultra-rapid vitrification using a nylon loop of 0.5 mm diameter. Oocytes that were vitrified using the loop survived at high rates and were fertilized following a small hole being made in the zona pellucida (69.8%) and developed to the blastocyst stage in culture (67.4%) at similar rates to that of oocytes that were not cryopreserved. Blastocysts resulting from oocytes vitrified using the nylon loop had similar development of the… CONTINUE READING