Vitrification of cryoelectron microscopy specimens revealed by high-speed photographic imaging.

@article{Kasas2003VitrificationOC,
  title={Vitrification of cryoelectron microscopy specimens revealed by high-speed photographic imaging.},
  author={S. Kasas and Georges Dumas and Giovanni Dietler and S. Catsicas and M Adrian},
  journal={Journal of microscopy},
  year={2003},
  volume={211 Pt 1},
  pages={
          48-53
        }
}
Cryoelectron microsopy is a widely used technique to observe biological material in an almost physiological, fully hydrated state. The sample is prepared for electron microsopy observation by quickly reducing its temperature to -180 degrees C. The high-speed cooling induces the formation of vitreous water, which preserves the sample conformation. However, the way vitrification occurs is still poorly understood. In order to better understand the phenomenon, we have used a stroboscopic device to… CONTINUE READING

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