Vitrification of cryoelectron microscopy specimens revealed by high‐speed photographic imaging

  title={Vitrification of cryoelectron microscopy specimens revealed by high‐speed photographic imaging},
  author={Sandor Kasas and Guy Dumas and Giovanni Dietler and Stefano Catsicas and Marc Adrian},
  journal={Journal of Microscopy},
Cryoelectron microsopy is a widely used technique to observe biological material in an almost physiological, fully hydrated state. The sample is prepared for electron microsopy observation by quickly reducing its temperature to −180 °C. The high‐speed cooling induces the formation of vitreous water, which preserves the sample conformation. However, the way vitrification occurs is still poorly understood. In order to better understand the phenomenon, we have used a stroboscopic device to… 

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Reference LPMV-ARTICLE-2003-011doi:10.1119/1.1523074View record in Web of Science Record created on 2007-06-20, modified on 2017-05-12