Vitellogenin in black turtle (Chelonia mydas agassizii): purification, partial characterization, and validation of an enzyme-linked immunosorbent assay for its detection.

@article{SifuentesRomero2006VitellogeninIB,
  title={Vitellogenin in black turtle (Chelonia mydas agassizii): purification, partial characterization, and validation of an enzyme-linked immunosorbent assay for its detection.},
  author={Itzel Sifuentes-Romero and Celia V{\'a}zquez-Boucard and Arturo Pedro Sierra-Beltr{\'a}n and Susan C. Gardner},
  journal={Environmental toxicology and chemistry},
  year={2006},
  volume={25 2},
  pages={477-85}
}
Black turtle plasmatic vitellogenin (VTG) was purified from 17beta-estradiol-induced males using ion-exchange chromatography. The isolated protein was identified as VTG by its glycolipoprotein nature and amino acid sequence homology with other vertebrate VTG. It was characterized as a 500-kDa dimer composed of two identical, 200- to 240-kDa monomers. Polyclonal antibodies raised against black turtle VTG showed high titer and specificity, as demonstrated by enzyme-linked immunosorbent assay and… CONTINUE READING