Vitellogenin in Zoarces viviparus: purification, quantification by ELISA and induction by estradiol-17 beta and 4-nonylphenol.

@article{Korsgaard1998VitellogeninIZ,
  title={Vitellogenin in Zoarces viviparus: purification, quantification by ELISA and induction by estradiol-17 beta and 4-nonylphenol.},
  author={Bodil Korsgaard and Knud Ladegaard Pedersen},
  journal={Comparative biochemistry and physiology. Part C, Pharmacology, toxicology \& endocrinology},
  year={1998},
  volume={120 1},
  pages={
          159-66
        }
}
  • B. Korsgaard, K. Pedersen
  • Published 1998
  • Biology, Medicine
  • Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology
Vitellogenin was isolated by gel filtration and ion-exchange chromatography from plasma of estradiol-treated male Zoarces viviparus. The purification of vitellogenin was followed through each step by native polyacrylamide gelelectrophoresis (PAGE). The purified vitellogenin was used to raise anti-vitellogenin antibodies in rabbits. The specificity of the affinity purified antibody raised against vitellogenin was assessed by Western blot analysis. No crossreactivity was observed with plasma from… Expand
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  • Biology, Medicine
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TLDR
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