Vitamin Requirements of Several Cellulolytic Rumen Bacteria

  title={Vitamin Requirements of Several Cellulolytic Rumen Bacteria},
  author={Harold William Scott and Burk A. Dehority},
  journal={Journal of Bacteriology},
  pages={1169 - 1175}
Scott, H. W. (Ohio Agricultural Experiment Station, Wooster), and B. A. Dehority. Vitamin requirements of several cellulolytic rumen bacteria. J. Bacteriol. 89:1169–1175. 1965.—Four strains of cellulolytic bacteria recently isolated from in vitro rumen fermentations were used in this study. Nine water-soluble vitamins were tested in single-deletion and single-addition plus biotin experiments, each with and without charcoal-extracted casein hydrolysate. Bacteroides succinogenes A3C and B21a… Expand
Magnesium requirement of some of the principal rumen cellulolytic bacteria.
Both strains of R. flavefaciens were found to have an absolute Mg requirement for cellulose degradation, and it seems unlikely that an in vivo deficiency of this element would occur. Expand
Characterization of Several Bovine Rumen Bacteria Isolated with a Xylan Medium
It is of interest that xylan-fermenting bacteria isolated from 10(-7) and 10(-8) dilutions of rumen contents by use of a xylan medium are similar to the xylan fermenters isolated at the same dilutions with a nonselective medium. Expand
Nutritional Requirements of Actinomyces Isolated from Rumen of Goat
Investigation of the nutritional requirements for the growth of Actinomyces sp. Expand
Ionized calcium requirement of rumen cellulolytic bacteria.
Based on reported concentrations of Ca(+2) in the rumen, it seems unlikely that an in vivo deficiency would occur for these bacteria, and the role of ionized calcium in growth and cellulose digestion is evaluated. Expand
Influence of Plant Phenolic Acids on Growth and Cellulolytic Activity of Rumen Bacteria
All of the cellulolytic strains (and Streptococcus bovis) showed at least a limited ability to hydrogenate hydroxycinnamic acids, with Ruminococcus spp. Expand
Pure and mixed continuous culture of two rumen anaerobes.
After 96 hr, however, Ruminococcus flavefaciens accounted for 20 to 40% of the total bacterial count, indicating Bacteroides ruminicola liberated sufficient methionine to support the growth of Ruminitis flave Faciens. Expand
Isolation and attempted introduction of sugar alcohol-utilizing bacteria in the sheep rumen.
In vitro experiments indicated that strain SS2/R5 grew less well in the presence of other rumen organisms, particularly ciliate protozoa, than in pure culture, indicating that sorbitol was absorbed rapidly from the rumen. Expand
Volatile Fatty Acid Requirements of Cellulolytic Rumen Bacteria
A gas chromatographic method was developed which could separate the isomers isovaleric and 2-methylbutyric acid. Subsequent analyses revealed that most commercially available samples of these acidsExpand
Characterization of rat cecum cellulolytic bacteria
Cellulose-degrading bacteria previously isolated from the ceca of rats have been characterized and identified, indicating the rat strains may form a distinct subgroup within the species B. succinogenes. Expand
Amino Acid and vitamin requirements of several bacteroides strains.
  • G. Quinto
  • Biology, Medicine
  • Applied microbiology
  • 1966
Nutritional studies were performed on nine Bacteroides strains, by use of the methodology and media of anaerobic rumen microbiology, and various strains produced maximal turbidity more slowly in media deficient in l-proline, glycine, l-glutamic acid, dl-serine, L-histidine hydrochloride, d-alanine, or l-cysteine hydro chloride, than in complete medium. Expand


The results indicate that most strains of ruminal bacteria can be grown in defined media, and suggest the relative importance of NH(4) (+) and volatile fatty acids and the relative lack of importance of organic nitrogen compounds such as amino acids in the nutrition of these bacteria. Expand
Observations on the Nutrition of Bacteroides Succinogenes—A Ruminal Cellulolytic Bacterium
The results indicate the relatively great ability of B. succinogenes to synthesize cellular constituents and, also, how well this bacterium is adapted to the environment of the rumen. Expand
Production of Vitamin B12 and Vitamin B12 Analogues by Pure Cultures of Ruminal Bacteria
Relatively little work has been reported in regard to the part played in the synthesis of these compounds by the individual species of micro-organisms obtained from the rumen and in general such studies have not included true or functional rumen organisms. Expand
Mutant strains of Escherichia coli unable to synthesize p-aminobenzoic acid.
The growth requirements of mutants obtained after irradiation with x-rays of the fungi Neurospora and Ophiostoma were studied to investigate the activity of various compounds related to PABA. Expand
The synthesis of methionine by suspensions of Escherichia coli.
The object of the present work was to obtain a simpler system for the synthesis of methionine than the whole growing culture, and one in which all the reactants could be clearly defined. Expand
The present report considers the nutritional characteristics of a cellulolytic bacterium belonging to the genus Buiyrivibrio as described by Bryant and Small (1956) and embraces the "less-cellulolytic rod" described by Hungate (1950). Expand
Interrelationships between folic acid and cobalamin in the synthesis of methionine by extracts of Escherichia coli.
Eggitt, P. W. R. & Norris, F. W. (1956). J. Sci. Fd Agric. 7, 493. Eggitt, P. W. R. & Ward, L. D. (1953). J. Sci. Fd Agric. 4, 569. Fieller, E. C. (1940). Suppl. J. Roy. 8atit. Soc. 7, 50. Friedman,Expand
The Amino Acid Composition of Proteins and Foods. Analytical Methods and Results
  • A. White
  • Computer Science
  • The Yale Journal of Biology and Medicine
  • 1945
The authors have done well to avoid dogmatism, and to indicate always that a given "conclusion" was one advanced by certain investigators in reporting their study; if, in the opinion of the authors, further investigation seems to be required, this has been stated. Expand
Alternative pathways for the methylation of homocysteine by Escherichia coli
  • Biochem. J
  • 1962