• DOI:10.1126/science.1218632
• Corpus ID: 8620984

Vitamin K2 Is a Mitochondrial Electron Carrier That Rescues Pink1 Deficiency

@article{Vos2012VitaminKI,
  title={Vitamin K2 Is a Mitochondrial Electron Carrier That Rescues Pink1 Deficiency},
  author={Melissa Vos and Giovanni Esposito and Janaka N. Edirisinghe and Sven Vilain and Dominik M Haddad and Jan R. Slabbaert and Stefanie Van Meensel and Onno Schaap and Bart de Strooper and Rangaswamy Meganathan and Vanessa Alexandra Morais and Patrik Verstreken},
  journal={Science},
  year={2012},
  volume={336},
  pages={1306 - 1310}
}

• M. Vos, G. Esposito, +9 authors P. Verstreken
• Published 8 June 2012
• Biology
• Science

Keeping Mitochondria in the Pink Pink1 is a mitochondrial kinase, and loss of Pink1 function in flies and mice results in the accumulation of inefficient mitochondria. In a screen for modifiers of the Parkinson-associated gene, pink1, Vos et al. (p. 1306, published online 10 May; see the Perspective by Bhalerao and Clandinin) identified the fruit fly homolog of UBIAD1, “Heix.” UBIAD1 was localized in mitochondria and was able to convert vitamin K1 into vitamin K2/menaquinone (MK-n, n the number of prenylgroups). In bacteria, vitamin K2/MK-n acts as an electron carrier in the membrane and, similarly, in Drosophila, mitochondrial vitamin K2 appeared to act as an electron carrier to facilitate adenosine triphosphate production. Fruit flies that lack heix showed severe mitochondrial defects that could be rescued by administering vitamin K2. Adding vitamin K rescues fruit flies bearing a mutation in a Parkinson’s disease gene homolog. Human UBIAD1 localizes to mitochondria and converts vitamin K1 to vitamin K2. Vitamin K2 is best known as a cofactor in blood coagulation, but in bacteria it is a membrane-bound electron carrier. Whether vitamin K2 exerts a similar carrier function in eukaryotic cells is unknown. We identified Drosophila UBIAD1/Heix as a modifier of pink1, a gene mutated in Parkinson’s disease that affects mitochondrial function. We found that vitamin K2 was necessary and sufficient to transfer electrons in Drosophila mitochondria. Heix mutants showed severe mitochondrial defects that were rescued by vitamin K2, and, similar to ubiquinone, vitamin K2 transferred electrons in Drosophila mitochondria, resulting in more efficient adenosine triphosphate (ATP) production. Thus, mitochondrial dysfunction was rescued by vitamin K2 that serves as a mitochondrial electron carrier, helping to maintain normal ATP production.