Vitamin D supplementation protects against bone loss following inhalant organic dust and lipopolysaccharide exposures in mice

  title={Vitamin D supplementation protects against bone loss following inhalant organic dust and lipopolysaccharide exposures in mice},
  author={Anand Dusad and Geoffrey M. Thiele and Lynell Warren Klassen and Dong Wang and Michael J. Duryee and Ted R. Mikuls and Elizabeth B Staab and Todd A. Wyatt and William W. West and Stephen J Reynolds and Debra J Romberger and Jill A. Poole},
  journal={Immunologic Research},
Abstract Systemic bone loss is associated with airway inflammatory diseases; yet, strategies to halt disease progression from inhalant exposures are not clear. Vitamin D might be a potentially protective approach against noxious respirable environmental exposures. We sought to determine whether vitamin D supplementation represents a viable lung- and bone-protective strategy following repetitive inhalant treatments with organic dust extract (ODE) or lipopolysaccharide (LPS) in mice. C57BL/5 mice… 

Systemic IL-6 Effector Response in Mediating Systemic Bone Loss Following Inhalation of Organic Dust.

  • Adam WellsD. Romberger J. Poole
  • Biology, Medicine
    Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research
  • 2017
Results show that the systemic IL- 6 effector pathway mediates bone deterioration induced by repetitive inhalant ODE exposures through an effect on osteoclasts, but a positive role for IL-6 in the airway was not demonstrated.

The Effect of Inhalant Organic Dust on Bone Health

Host factors, diet, and lipopolysaccharide/TLR4 signaling pathways play a significant role in explaining how inhalant organic dust exposures impact bone health and might lead to specific targeted therapeutic approaches.

Sex differences impact the lung-bone inflammatory response to repetitive inhalant lipopolysaccharide exposures in mice

Sex-specific differences exist in LPS-induced airway inflammatory consequences with significant differences found in bone quantity and quality parameters, and male mice demonstrated susceptibility to bone loss and female animals were protected, which was modulated by estrogen.

Age Impacts Pulmonary Inflammation and Systemic Bone Response to Inhaled Organic Dust Exposure

Collectively, age impacts the airway injury and systemic inflammatory and bone loss response to inhalant ODE, suggesting an altered and enhanced immunologic response in younger as compared to older counterparts.

Toll-Like Receptor 4 Signaling Pathway Mediates Inhalant Organic Dust-Induced Bone Loss

Results show that TLR2 and TLR4 pathways mediate ODE-induced airway inflammation, but bone deterioration consequences following inhalant ODE treatment is strongly dependent uponTLR4, which appears critical in regulating the lung-bone inflammatory axis to microbial component-enriched organic dust exposures.

Nutritional Factors in Occupational Lung Disease

Relevant nutrients that have been investigated in recent years include omega-3 polyunsaturated fatty acids, zinc, vitamin D, dairy products, and antioxidants, which have demonstrated the potential to prevent or modify the adverse outcomes associated with occupational exposures, primarily in preclinical studies.

High-dose 1,25-dihydroxyvitamin D supplementation elongates the lifespan of Huntington's disease transgenic mice.

The findings support the potential influence of vitamin D deficiency on the disease course and propose that vitamin D may be an effective supplementary treatment to beneficially influence clinical features of Huntington's disease.



Vitamin D Supplementation Protects against Bone Loss Associated with Chronic Alcohol Administration in Female Mice

Dietary VitD supplementation may prevent skeletal toxicity in chronic drinkers by normalizing calcium homeostasis, preventing apoptosis, and suppressing EtOH-induced increases in bone resorption.

Vitamin D Treatment Modulates Organic Dust–Induced Cellular and Airway Inflammatory Consequences

Collectively, vitamin D plays a role in modulating organic dust–induced airway inflammatory outcomes in cell culture and animal models and is associated with reduced tracheal epithelial cell PKCα and PKCε activity and whole lung TLR2 and TLR4 gene expression.

Repeated exposure to organic material alters inflammatory and physiological airway responses

It is concluded that individuals who are repeatedly exposed to organic material develop an adaptation to the effects of acute exposure to inhaled organic material.

Intranasal organic dust exposure-induced airway adaptation response marked by persistent lung inflammation and pathology in mice.

Intranasal exposure to DE results in significant lung inflammatory and pathological responses marked by a modulated innate immune response to single and repetitive dust exposures that is associated with PKC activity.

Chronic Obstructive Pulmonary Disease Is Associated with Low Levels of Vitamin D

COPD was associated with an increased risk of vitamin D deficiency, and important disease characteristics were significantly related to 25(OH)D levels.

Vitamin D levels, lung function, and steroid response in adult asthma.

In asthma, reduced vitamin D levels are associated with impaired lung function, increased AHR, and reduced GC response, suggesting that supplementation ofitamin D levels in patients with asthma may improve multiple parameters of asthma severity and treatment response.

Effect of vitamin D3 on asthma treatment failures in adults with symptomatic asthma and lower vitamin D levels: the VIDA randomized clinical trial.

Vitamin D3 did not reduce the rate of first treatment failure or exacerbation in adults with persistent asthma and vitamin D insufficiency, and findings do not support a strategy of therapeutic vitamin D3 supplementation in patients with symptomatic asthma.

Benefit–risk assessment of vitamin D supplementation

It is suggested that mean serum 25(OH)D levels of about 75 to 110 nmol/l provide optimal benefits for all investigated endpoints without increasing health risks.

αβ T cells and a mixed Th1/Th17 response are important in organic dust-induced airway disease.

  • J. PooleAngela M. Gleason T. Kielian
  • Biology, Medicine
    Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology
  • 2012

Toll-like receptor 2 regulates organic dust-induced airway inflammation.

It is demonstrated that the TLR 2 pathway is important in regulating swine facility organic dust-induced airway inflammation, which suggests the importance of TLR2 agonists in mediating large animal farming- induced airway inflammatory responses.