Vitamin C matters: increased oxidative stress in cultured human aortic endothelial cells without supplemental ascorbic acid.

@article{Smith2002VitaminCM,
  title={Vitamin C matters: increased oxidative stress in cultured human aortic endothelial cells without supplemental ascorbic acid.},
  author={Anthony RB Smith and Francesco Visioli and Tory M. Hagen},
  journal={FASEB journal : official publication of the Federation of American Societies for Experimental Biology},
  year={2002},
  volume={16 9},
  pages={1102-4}
}
Because standard culture media for human aortic endothelial cells (HAEC) do not contain vitamin C, we hypothesized that HAEC may be under significant oxidative insult compared with the situation in vivo. To assess parameters of oxidative stress, intracellular vitamin C, glutathione (GSH), GSH/GSSG, and NAD(P)H/NAD(P)+ ratios, as well as oxidant appearance and oxidative damage, were measured in HAEC with or without vitamin C addition. The effect of vitamin C on eNOS activity was also determined… CONTINUE READING