Visualizing the assembly of human Rad51 filaments on double-stranded DNA.

  title={Visualizing the assembly of human Rad51 filaments on double-stranded DNA.},
  author={Tekkatte Krishnamurthy Prasad and Caitlyn C Yeykal and Eric C Greene},
  journal={Journal of molecular biology},
  volume={363 3},
Rad51 is the core component of the eukaryotic homologous recombination machinery and assembles into extended nucleoprotein filaments on DNA. To study the dynamic behavior of Rad51 we have developed a single-molecule assay that relies on a combination of hydrodynamic force and microscale diffusion barriers to align individual DNA molecules on the surface of a microfluidic sample chamber that is coated with a lipid bilayer. When visualized with total internal reflection fluorescence microscopy… CONTINUE READING

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