Visualizing the Ca2+-dependent activation of gelsolin by using synchrotron footprinting.

  title={Visualizing the Ca2+-dependent activation of gelsolin by using synchrotron footprinting.},
  author={Janna G. Kiselar and Paul A. Janmey and Steven C. Almo and Mark R. Chance},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={100 7},
Radiolytic protein footprinting with a synchrotron source is used to reveal detailed structural changes that occur in the Ca(2+)-dependent activation of gelsolin. More than 80 discrete peptides segments within the structure, covering 95% of the sequence in the molecule, were examined by footprinting and mass spectrometry for their solvent accessibility as a function of Ca(2+) concentration in solution. Twenty-two of the peptides exhibited detectable oxidation; for seven the oxidation extent was… CONTINUE READING

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