Visualizing single DNA-bound proteins using DNA as a scanning probe

@article{Noom2007VisualizingSD,
  title={Visualizing single DNA-bound proteins using DNA as a scanning probe},
  author={M. C. Noom and B. V. D. Broek and J. V. Mameren and G. Wuite},
  journal={Nature Methods},
  year={2007},
  volume={4},
  pages={1031-1036}
}
  • M. C. Noom, B. V. D. Broek, +1 author G. Wuite
  • Published 2007
  • Biology, Medicine
  • Nature Methods
  • Many biological processes involve enzymes moving along DNA. Such motion might be impeded by DNA-bound proteins or DNA supercoils. Current techniques are incapable of directly measuring forces that such 'roadblocks' might impose. We constructed a setup with four independently moveable optical traps, allowing us to manipulate two DNA molecules held between beads. By tightly wrapping one DNA around the other, we created a probe that can be scanned along the contour of the second DNA. We found that… CONTINUE READING
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    References

    SHOWING 1-10 OF 38 REFERENCES
    Enzyme-mediated DNA looping.
    • 113
    STM and AFM images of nucleosome DNA under water.
    • 83