Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins

@article{Miesenbock1998VisualizingSA,
  title={Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins},
  author={Gero Miesenböck and Dino A. De Angelis and James E. Rothman},
  journal={Nature},
  year={1998},
  volume={394},
  pages={192-195}
}
In neural systems, information is often carried by ensembles of cells rather than by individual units. Optical indicators provide a powerful means to reveal such distributed activity, particularly when protein-based and encodable in DNA: encodable probes can be introduced into cells, tissues, or transgenic organisms by genetic manipulation, selectively expressed in anatomically or functionally defined groups of cells, and, ideally, recorded in situ, without a requirement for exogenous cofactors… 

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Intracellular pH measurements in vivo using green fluorescent protein variants.

  • K. Nehrke
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TLDR
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...

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