Visualizing monolayers with a water-soluble fluorophore to quantify adsorption, desorption, and the double layer.

Abstract

Contrast in confocal microscopy of phase-separated monolayers at the air-water interface can be generated by the selective adsorption of water-soluble fluorescent dyes to disordered monolayer phases. Optical sectioning minimizes the fluorescence signal from the subphase, whereas convolution of the measured point spread function with a simple box model of… (More)
DOI: 10.1073/pnas.1419033112

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