Visualizing looping of two endogenous genomic loci using synthetic zinc‐finger proteins with anti‐FLAG and anti‐HA frankenbodies in living cells

@article{Liu2021VisualizingLO,
  title={Visualizing looping of two endogenous genomic loci using synthetic zinc‐finger proteins with anti‐FLAG and anti‐HA frankenbodies in living cells},
  author={Yang Liu and Ning Zhao and Masato T. Kanemaki and Yotaro Yamamoto and Yoshifusa Sadamura and Yuma Ito and Makio Tokunaga and Timothy J. Stasevich and Hiroshi Kimura},
  journal={Genes to Cells},
  year={2021},
  volume={26},
  pages={905 - 926}
}
In eukaryotic nuclei, chromatin loops mediated through cohesin are critical structures that regulate gene expression and DNA replication. Here we demonstrate a new method to visualize endogenous genomic loci using synthetic zinc-finger proteins harboring repeat epitope tags (ZF probes) for signal amplification via binding of tag-specific intracellular antibodies, or frankenbodies, fused with fluorescent proteins. We achieve this in two steps. First, we develop an anti-FLAG frankenbody that can… 

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