Visualizing cellular processes at the molecular level by cryo-electron tomography.

Abstract

The cellular landscape rapidly changes throughout the biological processes that transpire within a cell. For example, the cytoskeleton is remodeled within fractions of a second. Therefore, reliable structural analysis of the cell requires approaches that allow for instantaneous arrest of functional states of a given process while offering the best possible preservation of the delicate cellular structure. Electron tomography of vitrified but otherwise unaltered cells (cryo-ET) has proven to be the method of choice for three-dimensional (3D) reconstruction of cellular architecture at a resolution of 4-6 nm. Through the use of cryo-ET, the 3D organization of macromolecular complexes and organelles can be studied in their native environment in the cell. In this Commentary, we focus on the application of cryo-ET to study eukaryotic cells - in particular, the cytoskeletal-driven processes that are involved in cell movements, filopodia protrusion and viral entry. Finally, we demonstrate the potential of cryo-ET to determine structures of macromolecular complexes in situ, such as the nuclear pore complex.

DOI: 10.1242/jcs.060111

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@article{BenHarush2010VisualizingCP, title={Visualizing cellular processes at the molecular level by cryo-electron tomography.}, author={Kfir Ben-Harush and Tal Maimon and Israel Patla and Elizabeth Villa and Ohad Medalia}, journal={Journal of cell science}, year={2010}, volume={123 Pt 1}, pages={7-12} }