Visualizing cell structure and function with point-localization superresolution imaging.

@article{Sengupta2012VisualizingCS,
  title={Visualizing cell structure and function with point-localization superresolution imaging.},
  author={Prabuddha Sengupta and Schuyler B. Van Engelenburg and Jennifer Lippincott-Schwartz},
  journal={Developmental cell},
  year={2012},
  volume={23 6},
  pages={1092-102}
}
Fundamental to the success of cell and developmental biology is the ability to tease apart molecular organization in cells and tissues by localizing specific proteins with respect to one another in a native cellular context. However, many key cellular structures (from mitochondrial cristae to nuclear pores) lie below the diffraction limit of visible light, precluding analysis of their organization by conventional approaches. Point-localization superresolution microscopy techniques, such as PALM… CONTINUE READING
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