Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression

@article{SakaueSawano2008VisualizingSD,
  title={Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression},
  author={Asako Sakaue-Sawano and Hiroshi Kurokawa and Toshifumi Morimura and Aki Hanyu and Hiroshi Hama and Hatsuki Osawa and Saori Kashiwagi and Kiyoko Fukami and Takaki Miyata and Hiroyuki Miyoshi and Takeshi Imamura and Masaharu Ogawa and Hisao Masai and Atsushi Miyawaki},
  journal={Cell},
  year={2008},
  volume={132},
  pages={487-498}
}
The cell-cycle transition from G1 to S phase has been difficult to visualize. We have harnessed antiphase oscillating proteins that mark cell-cycle transitions in order to develop genetically encoded fluorescent probes for this purpose. These probes effectively label individual G1 phase nuclei red and those in S/G2/M phases green. We were able to generate cultured cells and transgenic mice constitutively expressing the cell-cycle probes, in which every cell nucleus exhibits either red or green… Expand
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