Visualization of von Willebrand factor multimers by immunoenzymatic stain using avidin-biotin peroxidase complex.

@article{Aihara1986VisualizationOV,
  title={Visualization of von Willebrand factor multimers by immunoenzymatic stain using avidin-biotin peroxidase complex.},
  author={Masayuki Aihara and Yoshiko Sawada and Keiichi Ueno and Satoshi Morimoto and Yuich Yoshida and M de Serres and Herbert A Cooper and Robert H. Wagner},
  journal={Thrombosis and haemostasis},
  year={1986},
  volume={55 2},
  pages={263-7}
}
A technique for the detection of von Willebrand factor multimers separated by discontinuous SDS agarose electrophoresis has been developed using non-radioactive compounds. The multimeric patterns were visualized by monospecific anti-human vWF:Ag followed by incubation with biotinylated antibody. After addition of avidin-biotin-peroxidase complex, the peroxidase activity was detected by 4-chloro-1-naphthol, giving sharp bands with a clear background. By this method, the differences of vWF:Ag… CONTINUE READING

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