Visualization of vacuoles in Aspergillus oryzae by expression of CPY-EGFP.

  title={Visualization of vacuoles in Aspergillus oryzae by expression of CPY-EGFP.},
  author={Mamoru Ohneda and M. Arioka and Harushi Nakajima and Katsuhiko Kitamoto},
  journal={Fungal genetics and biology : FG & B},
  volume={37 1},
Vacuolar carboxypeptidase Y (CPY) from Aspergillus nidulans was used to construct a CPY-EGFP fusion protein and expressed in A. oryzae to study vacuolar morphology and functions in A. oryzae. While the fluorescence of EGFP was barely detectable in A. oryzae expressing CPY-EGFP grown under normal conditions at pH 5-6, the increase in pH of the growth medium towards alkalinity restored the fluorescence. In accordance with such an observation, the fluorescence of CPY-EGFP fusion protein in cell… CONTINUE READING

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