Visualization of tyrosinase activity in melanoma cells by a BODIPY-based fluorescent probe.

  title={Visualization of tyrosinase activity in melanoma cells by a BODIPY-based fluorescent probe.},
  author={Tae-Il Kim and Jihye Park and Seonhwa Park and Yongdoo Choi and Youngmi Kim},
  journal={Chemical communications},
  volume={47 47},
We have presented a fluorescent probe 1 that exhibits a fluorescence turn-on signal upon reaction with tyrosinase, and we show that it is readily employed for the assessment of tyrosinase activity and tyrosinase inhibitor activity in buffered aqueous solution, and further utilized for the visualization of endogenous tyrosinase activity in living melanoma cells. 
A turn-on fluorescent probe for detection of tyrosinase activity.
A fluorescent probe that exhibits a fluorescence turn-on signal upon reaction with tyrosinase is presented and it is shown that it can be readily employed for the assessment of tyros in buffered aqueous solution.
A Bioluminogenic Probe for Monitoring Tyrosinase Activity.
A bioluminogenic probe based on luciferin was designed and synthesized and efficient in assessing tyrosinase activity in a buffered aqueous solution and in measuring endogenous tyros in melanoma cells.
A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells.
The nontoxic probe Tyro-1 provides information about H2O2-mediated upregulation of tyrosinase through Cellular imaging through cellular imaging, and its two-photon imaging ability makes it a noninvasive tool for validating the expression of tyosinase in the live cells.
Highly selective two-photon fluorescent off-on probes for imaging tyrosinase activity in living cells and tissues.
A coumarin-based two-photon (TP) fluorescent off-on probe has been developed for detecting tyrosinase activity. High selectivity, sensitivity and biocompatibility enable the probes to successfully
A highly sensitive and selective fluorescence off-on probe for the detection of intracellular endogenous tyrosinase activity.
A resorufin-based highly sensitive and selective fluorescence off-on probe with a new recognition moiety for tyrosinase is developed, and applied to detect and image endogenous tyrosinase activity in
A two-photon fluorescent probe for intracellular detection of tyrosinase activity.
The two-photon turn-on fluorescent probe NHU was synthesized to optically detect tyrosinase activity in vitro and in melanoma cells and releases the highly fluorescent product 6-acyl-N-methyl-2-naphthylamine (AAN).
Fast tyrosinase detection in early stage melanoma with nanomolar sensitivity using a naphthalimide-based fluorescent read-out probe
Naphthalimide-based red-emitting fluorescent read-out probes are developed for expeditious and sensitive detection of tyrosinase activity in live-cells and are highly suitable for early melanoma detection.
A novel hydrosoluble near-infrared fluorescent probe for specifically monitoring tyrosinase and application in a mouse model.
A novel hydrosoluble near-infrared (NIR) fluorescent probe that could specifically identify tyrosinase has been successfully constructed and applied to the diagnosis of melanoma in a xenogeneic mouse model.


A near-infrared fluorescent probe for monitoring tyrosinase activity.
A new cyanine-based near-infrared fluorescent probe has been developed for monitoring tyrosinase activity and its application in high-performance liquid chromatography has been proposed.
Novel tyramide‐based tyrosinase assay for the detection of melanoma cells in cytological preparations
Results suggest that this simple, fast, and inexpensive assay is a sensitive and specific method for detection of melanoma cells in cytology specimens, and may be a useful ancillary procedure for the resolution of challenging melanoma cases.
Synthesis of a new water-soluble oligo(phenylenevinylene) containing a tyrosine moiety for tyrosinase activity detection.
A new water-soluble oligo(phenylenevinylene) containing a tyrosine unit (OPV-Tyr) was synthesized as a fluorescent probe to optically detect tyrosinase activity. Upon the addition of tyrosinase, the
The chemistry of fluorescent bodipy dyes: versatility unsurpassed.
The Bodipy family, first developed as luminescent tags and laser dyes, has become a cornerstone for these new applications and the near future looks extremely bright for "porphyrin's little sister".
Highly sensitive fluorescence probes for nitric oxide based on boron dipyrromethene chromophore-rational design of potentially useful bioimaging fluorescence probe.
The development of DAMBO-P(H), a highly sensitive fluorescence probe for nitric oxide (NO), was developed based on the BODIPY chromophore and the change of the fluorescence intensity was found to be controlled by an intramolecular photoinduced electron transfer (PeT) mechanism.
Novel optical pH-sensor based on a boradiaza-indacene derivative
Abstract A pH-optode is described based on 1, 3, 5, 7-tetramethyl-8-(4-dimethylamino)-4-difluorobora-3a, 4a-diaza-(s)-indacene (1) immobilized in a hydrogel matrix. Its uncharged form is
Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future
  • Y. Kim, H. Uyama
  • Chemistry, Medicine
    Cellular and Molecular Life Sciences CMLS
  • 2005
This article overviews the various inhibitors obtained from natural and synthetic sources with their industrial importance, and examines the role of tyrosinase in melanin biosynthesis and its role in dermatological disorders.
Inhibitory effect of miconazole on melanogenesis.
Results indicate that MIC may be a useful inhibitor of melanogenesis in B16 cells and suggest that it may have beneficial effects in the treatment of hyperpigmentation disorders such as ephelis and melasma.
Cyclic AMP promotes a peripheral distribution of melanosomes and stimulates melanophilin/Slac2‐a and actin association
It is proposed that the stimulation of the interaction between melanophilin/Slac2‐a and actin would allow the rapid accumulation of melanosomes in the actin‐rich region of the dendrite extremities.
Mushroom tyrosinase: recent prospects.
A study on the importance of tyrosinase, especially that derived from mushroom, and describes its biochemical character and inhibition and activation by the various chemicals obtained from natural and synthetic origins with its clinical and industrial importance in the recent prospects.