Visualization of transcription sites at the electron microscope.

@article{Trentani2003VisualizationOT,
  title={Visualization of transcription sites at the electron microscope.},
  author={Andrea Trentani and Pilar S Testillano and Ma Carmen Risue{\~n}o and Marco Biggiogera},
  journal={European journal of histochemistry : EJH},
  year={2003},
  volume={47 3},
  pages={195-200}
}
In order to localize at EM level the sites of transcription of both pre-mRNA and pre-rRNA, we have detected the DNA/RNA hybrid molecules and m3Gcapped structures by means of specific antibodies after short bromo-uridine (BrU) incorporation. In addition, the sections have been stained by a selective RNA stain, terbium citrate. Our data indicate that perichromatin fibrils incorporate BrU and are labeled by the anti-hybrid probe; this supports the idea that they are the pre-mRNA transcription… CONTINUE READING
13 Citations
38 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 13 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 38 references

Cerdido A, de Cárcer G.The functional organization of the nucleolus in plant cells

  • FJ Medina
  • Eur J Histochem
  • 2000

Similar Papers

Loading similar papers…