Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis.

@article{Makino2015VisualizationOT,
  title={Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis.},
  author={Asami Makino and Mitsuhiro Abe and Motohide Murate and Takehiko Inaba and Neval Yilmaz and Françoise Hullin-Matsuda and Takuma Kishimoto and Nicole L Schieber and Tomohiko Taguchi and Hiroyuki Arai and Gregor Anderluh and Robert G. Parton and Toshihide Kobayashi},
  journal={FASEB journal : official publication of the Federation of American Societies for Experimental Biology},
  year={2015},
  volume={29 2},
  pages={
          477-93
        }
}
Sphingomyelin (SM) is a major sphingolipid in mammalian cells and is reported to form specific lipid domains together with cholesterol. However, methods to examine the membrane distribution of SM are limited. We demonstrated in model membranes that fluorescent protein conjugates of 2 specific SM-binding toxins, lysenin (Lys) and equinatoxin II (EqtII), recognize different membrane distributions of SM; Lys exclusively binds clustered SM, whereas EqtII preferentially binds dispersed SM. Freeze… CONTINUE READING
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