Visualization of the Xenopus primordial germ cells using a green fluorescent protein controlled by cis elements of the 3′ untranslated region of the DEADSouth gene

@article{Kataoka2006VisualizationOT,
  title={Visualization of the Xenopus primordial germ cells using a green fluorescent protein controlled by cis elements of the 3′ untranslated region of the DEADSouth gene},
  author={Kensuke Kataoka and Takuhiro Yamaguchi and Hidehumi Orii and Akira Tazaki and Kenji Watanabe and Makoto Mochii},
  journal={Mechanisms of Development},
  year={2006},
  volume={123},
  pages={746-760}
}
We succeeded in visualization of the primordial germ cells (PGCs) in a living Xenopus embryo. The mRNA of the reporter Venus protein, fused to the 3' untranslated region (UTR) of DEADSouth, which is a component of the germ plasm in Xenopus eggs, was microinjected into the vegetal pole of fertilized eggs and then the cells with Venus fluorescence were monitored during development. The behavior of the cells was identical to that previously described for PGCs. Almost all Venus-expressing cells… CONTINUE READING
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