Visualization of the ER-to-cytosol dislocation reaction of a type I membrane protein.

@article{Fiebiger2002VisualizationOT,
  title={Visualization of the ER-to-cytosol dislocation reaction of a type I membrane protein.},
  author={Edda Fiebiger and Craig M. Story and Hidde L. Ploegh and Domenico Tortorella},
  journal={The EMBO journal},
  year={2002},
  volume={21 5},
  pages={1041-53}
}
The human cytomegalovirus gene products US2 and US11 induce proteasomal degradation of MHC class I heavy chains. We have generated an enhanced green fluorescent protein-class I heavy chain (EGFP-HC) chimeric molecule to study its dislocation and degradation in US2- and US11-expressing cells. The EGFP-HC fusion is stable in control cells, but is degraded rapidly in US2- or US11-expressing cells. Proteasome inhibitors induce in a time-dependent manner the accumulation of EGFP-HC molecules in US2… CONTINUE READING

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