Visualization of single RNA transcripts in situ.

  title={Visualization of single RNA transcripts in situ.},
  author={Andrea M. Femino and Fredric S. Fay and Kevin E. Fogarty and Robert H. Singer},
  volume={280 5363},
Fluorescence in situ hybridization (FISH) and digital imaging microscopy were modified to allow detection of single RNA molecules. Oligodeoxynucleotide probes were synthesized with five fluorochromes per molecule, and the light emitted by a single probe was calibrated. Points of light in exhaustively deconvolved images of hybridized cells gave fluorescent intensities and distances between probes consistent with single messenger RNA molecules. Analysis of beta-actin transcription sites after… 

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