Visualization of sialoglycoproteins in polyacrylamide gels by acidic ninhydrin reaction.

@article{Yao1990VisualizationOS,
  title={Visualization of sialoglycoproteins in polyacrylamide gels by acidic ninhydrin reaction.},
  author={Kouakou Yao and Toshihiko Ubuka and Noriyoshi Masuoka and Masahiro Kinuta and Jiro Ohta and Kozo Ishino},
  journal={Acta medica Okayama},
  year={1990},
  volume={44 2},
  pages={65-70}
}
A new method for staining sialoglycoproteins in polyacrylamide gel after disc electrophoresis is described. The method utilizes the reaction of sialic acids with an acidic ninhydrin reagent which yields a stable color with an absorption maximum at 470 nm. After electrophoresis, the polyacrylamide gel is placed in a test tube and heated with 5 ml of the acidic ninhydrin reagent for 10 min in a boiling water bath. Sialoglycoproteins are detected as brown bands. No additional procedure such as… CONTINUE READING