Visualization of microtubule growth in living platelets reveals a dynamic marginal band with multiple microtubules.

@article{PatelHett2008VisualizationOM,
  title={Visualization of microtubule growth in living platelets reveals a dynamic marginal band with multiple microtubules.},
  author={Sunita Patel-Hett and Jennifer L. Richardson and Harald Schulze and Ksenija Drabek and Natasha A Isaac and Karin M. Hoffmeister and Ramesh A. Shivdasani and Jeannette Chlo{\"e} Bulinski and Niels Galjart and John H. Hartwig and Joseph E. Italiano},
  journal={Blood},
  year={2008},
  volume={111 9},
  pages={
          4605-16
        }
}
The marginal band of microtubules maintains the discoid shape of resting blood platelets. Although studies of platelet microtubule coil structure conclude that it is composed of a single microtubule, no investigations of its dynamics exist. In contrast to previous studies, permeabilized platelets incubated with GTP-rhodamine-tubulin revealed tubulin incorporation at 7.9 (+/- 1.9) points throughout the coil, and anti-EB1 antibodies stained 8.7 (+/- 2.0) sites, indicative of multiple free… 

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