Visualization of lipoprotein receptors by ligand blotting.

  title={Visualization of lipoprotein receptors by ligand blotting.},
  author={Thomas O. Daniel and Wolfgang Johann Schneider and Joseph L Goldstein and Michael Scott Brown},
  journal={The Journal of biological chemistry},
  volume={258 7},
This paper describes the visualization of the low density lipoprotein (LDL) receptor by ligand blotting. Preparations of detergent-solubilized membranes are subjected to one- or two-dimensional sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, after which the proteins are transferred to nitrocellulose paper. The paper is incubated with native LDL and then with an 125I-labeled antibody against LDL, and the bound antibody is visualized by autoradiography. The success of LDL… CONTINUE READING


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