Visualization of labile zinc and its role in apoptosis of primary airway epithelial cells and cell lines.

  title={Visualization of labile zinc and its role in apoptosis of primary airway epithelial cells and cell lines.},
  author={Ai Q. Truong-Tran and Richard E. Ruffin and Peter David Zalewski},
  journal={American journal of physiology. Lung cellular and molecular physiology},
  volume={279 6},
The respiratory epithelium is vulnerable to noxious substances, resulting in the shedding of cells and decreased protection. Zinc (Zn), an antioxidant and cytoprotectant, can suppress apoptosis in a variety of cells. Here we used the novel Zn-specific fluorophore Zinquin to visualize and quantify labile intracellular Zn in respiratory epithelial cells. Zinquin fluorescence in isolated ciliated tracheobronchial epithelial cells and intact epithelium from sheep and pigs revealed an intense… 

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It is concluded that zinc is a vital factor in the lung epithelium that protects against death receptor-mediated apoptosis and barrier dysfunction and facilitates future studies aimed at developing therapeutic strategies to prevent acute lung injury.



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