Visualization of human immunodeficiency virus protease inhibition using a novel Förster resonance energy transfer molecular probe.

  title={Visualization of human immunodeficiency virus protease inhibition using a novel F{\"o}rster resonance energy transfer molecular probe.},
  author={Sha Jin and Erika M Ellis and Jithesh V. Veetil and Huantong Yao and Kaiming Ye},
  journal={Biotechnology progress},
  volume={27 4},
The in vivo high-throughput screening (HTS) of human immunodeficiency virus (HIV) protease inhibitors is a significant challenge because of the lack of reliable assays that allow the visualization of HIV targets within living cells. In this study, we developed a new molecular probe that utilizes the principles of Förster resonance energy transfer (FRET) to visualize HIV-1 protease inhibition within living cells. The probe is constructed by linking two fluorescent proteins: AcGFP1 (a mutant… CONTINUE READING


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