• Biology, Medicine
  • Published in
    European journal of cell…
    1985

Visualization of epidermal growth factor receptor in cryosections of cultured A431 cells by immuno-gold labeling.

@article{Boonstra1985VisualizationOE,
  title={Visualization of epidermal growth factor receptor in cryosections of cultured A431 cells by immuno-gold labeling.},
  author={Johannes Boonstra and P van Maurik and Libert Defize and Siegfried W. de Laat and J. L. M. Leunissen and A. J. Verkley},
  journal={European journal of cell biology},
  year={1985},
  volume={36 2},
  pages={
          209-16
        }
}
Cryo-ultramicrotomy in combination with immuno-gold labeling has been demonstrated to present a powerful tool in the visualization of extra- and intracellular located antigens. We have applied this method to localize epidermal growth factor (EGF) receptor in cultured A431 human epidermoid carcinoma cells. However, both the labeling efficiency, maintenance of antigenicity, and the recognizability of the ultrastructure in cryosections are highly dependent upon the fixation procedures. Using 125I… CONTINUE READING

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