Visualization of early events in tumor formation of eGFP‐transfected rat colon cancer cells in liver

  title={Visualization of early events in tumor formation of eGFP‐transfected rat colon cancer cells in liver},
  author={Olaf R. F. Mook and Jan van Marle and Heleen Vreeling-Sindel{\'a}rov{\'a} and Remmet Jonges and Wilma M. Frederiks and Cornelius J. F. Van Noorden},
Colon cancer preferentially metastasizes to the liver. To determine cellular backgrounds of this preference, we generated an enhanced green fluorescent protein (eGFP)‐expressing rat adenocarcinoma cell line (CC531s) that forms metastases in rat liver after administration to the portal vein. Intravital videomicroscopy (IVVM) was used to visualize early events in the development of tumors in livers of live animals from the time of injection of the cancer cells up to 4 days afterward. Based on… 

Loss of a reporter gene for green fluorescent protein during tumor progression suggests the recruitment of host cells in rats with experimentally induced colon cancer.

It is postulated that tumor growth did not occur exclusively as a result of the division of the injected cells, but also involved recruitment of host cells in the development of tumors.

Metastatic tumor cell arrest in the liver–lumen occlusion and specific adhesion are not exclusive

It is demonstrated that lumen occlusion alone, as determined by in vivo microscopy, is insufficient to establish stable tumor cell arrest of colon carcinoma cells in metastatic target organs and does therefore not rule out the requirement of specific adhesive interactions for tumor cell Arrest in the microcirculation.

Visualizing portal vein metastatic trafficking to the liver with green fluorescent protein-expressing tumor cells.

The critical role of the portal vein in metastasis to the liver is demonstrated by visualizing the trafficking of metastatic cells targeting the liver via the portals vein using green fluorescent protein (GFP)-expressing cancer cells.

Rapid Extravasation and Establishment of Breast Cancer Micrometastases in the Liver Microenvironment

The use of two-photon microscopy is presented to directly compare extravasation times in metastatic sites using the same tumor cell line and highlight the differences in early events and metastatic patterns between two important secondary sites of breast cancer progression with implications for future therapy.

Interactions between rat colon carcinoma cells and Kupffer cells during the onset of hepatic metastasis

Early interaction of KCs, NK and CC531s colon carcinoma cells in a syngeneic rat model by confocal laser scanning microscopy showed Surviving cancer cells were primarily located close to the Glisson capsule, suggesting that metastasis would initiate from this region.

Real Time Metastatic Route Tracking of Orthotopic PC‐3‐GFP Human Prostate Cancer Using Intravital Imaging

Metastatic cells were observed migrating superiorly and inferiorly from the primary tumor as well as in lymphatic channels and trafficking in various organ systems demonstrating that PC‐3 has multiple metastatic routes similar to hormone‐independent advanced‐stage prostate cancer in the clinic.

In vivo tumor cell adhesion in the pulmonary microvasculature is exclusively mediated by tumor cell - endothelial cell interaction

In contrast to reports of earlier studies that metastatic tumor cell adhesion occurs through integrin mediated binding of extracellular matrix proteins in liver, in the lung, the continuously lined endothelium appears to be specifically targeted by circulating tumor cells.

In vivo cell biology of cancer cells visualized with fluorescent proteins.

  • R. Hoffman
  • Biology
    Current topics in developmental biology
  • 2005

In Vivo Cellular Imaging Using Fluorescent Proteins

Recent developments of detailed procedures for using shell-less chick embryos, coupled with fl uorescent labeling of cancer cells and/or chick vasculature, to study cancer cell migration and metastasis in vivo are presented.

Organ-specific metastatic tumor cell adhesion and extravasation of colon carcinoma cells with different metastatic potential.

It is indicated that colon carcinoma cells can arrest in target organs without size restriction, and cell adhesion of circulating tumor cells occurred in metastatic target organs only, likely attributable to specific interactions.



Real‐time observation of micrometastasis formation in the living mouse liver using a green fluorescent protein gene–tagged rat tongue carcinoma cell line

The results suggest that micrometastasis formation by LM‐EGFP cells consists of initial tumor cell arrest due to size constraints of the vessel, stable attachment to subsinusoidal basement membrane and subsequent intravascular growth before extravasation.

In vivo microscopy of hepatic metastases: Dynamic observation of tumor cell invasion and interaction with Kupffer cells

In vivo microscopic images showed that Kupffer cells are not only attracted to tumor cells in the hepatic circulation but also have the ability to phagocytose those tumor cells.

Visualization of the metastatic process by green fluorescent protein expression.

The visualization of the cancer metastatic process in live tissue in vivo by green fluorescent protein (GFP) expression indicates that initial steps of the metastatic cascade influence the subsequent progression of metastasis.

Cell motility of tumor cells visualized in living intact primary tumors using green fluorescent protein.

This model develops a model that directly examines the motility of metastatic primary tumor cells in situ and describes and determined the behavioral phenotype of metastatics and nonmetastatic tumor cells, which will allow the effects of genetic manipulations or therapeutic treatments on this phenotype to be determined.

Quantitative detection of lac-Z-transfected CC531 colon carcinoma cells in an orthotopic rat liver metastasis model

An orthotopic liver metastases model based on CC531 rat colon adenocarcinoma cells which were transfected with a β-galactosidase gene as marker to facilitate their detection is established and it is envisaged that the model will have applications for various therapeutic strategies.

Video microscopy of tumor metastasis: using the green fluorescent protein (GFP) gene as a cancer-cell-labeling system

  • Z. KanT. Liu
  • Biology, Medicine
    Clinical & Experimental Metastasis
  • 2004
Video microscopy allows dynamic observation of cancer-cell activity in the microcirculation of live animals. However, observation of cancer invasion and metastasis in situ has never been successful

Intravascular origin of metastasis from the proliferation of endothelium-attached tumor cells: a new model for metastasis

It is shown that in the lung, metastasis is initiated by attachment of tumor cells to the vascular endothelium and that hematogenous metastasis originates from the proliferation of attached intravascular tumor cells rather than from extravasated ones.

Early interactions of cancer cells with the microvasculature in mouse liver and muscle during hematogenous metastasis: videomicroscopic analysis

In this study intravital videomicroscopy has provided a valuable clarification of the interactions of cancer cells with vessel walls during metastasis: large membrane-enclosed fragments became ‘pinched off’ from arrested cells, in both liver and muscle, often within minutes or even seconds of arrest.

Cellular expression of green fluorescent protein, coupled with high-resolution in vivo videomicroscopy, to monitor steps in tumor metastasis.

The advantages of GFP-expressing cells, coupled with real-time high resolution videomicroscopy, for long-term in vivo studies to visualize and quantify sequential steps of the metastatic process are demonstrated.

Kupffer cells and pit cells are not effective in the defense against experimentally induced colon carcinoma metastasis in rat liver

The low activation status of K upffer cells both in terms of production of reactive oxygen species and Ia-antigen expression and the absence of significant numbers of pit cells at tumor sites suggest that Kupffer cells and pit cells do not play a significant role in advanced stages of tumor growth.