Visualization of early chromosome condensation

  title={Visualization of early chromosome condensation},
  author={Natashe Kireeva and Margot Lakonishok and Igor Kireev and Tatsuya Hirano and Andrew S Belmont},
  journal={The Journal of Cell Biology},
  pages={775 - 785}
Current models of mitotic chromosome structure are based largely on the examination of maximally condensed metaphase chromosomes. Here, we test these models by correlating the distribution of two scaffold components with the appearance of prophase chromosome folding intermediates. We confirm an axial distribution of topoisomerase IIalpha and the condensin subunit, structural maintenance of chromosomes 2 (SMC2), in unextracted metaphase chromosomes, with SMC2 localizing to a 150-200-nm-diameter… CONTINUE READING