Visualization of chromosomal domains with boundary element-associated factor BEAF-32

  title={Visualization of chromosomal domains with boundary element-associated factor BEAF-32},
  author={Keji Zhao and Craig M. Hart and U. K. Laemmli},
We have purified two proteins from Drosophila that bind to the scs' boundary element of the hsp70 domain at locus 87A7. Their palindromic binding sites (CGATA-TATCG) symmetrically abut the previously mapped hypersensitive site of scs'. We have cloned a cDNA for one of these proteins, BEAF-32 (boundary element-associated factor of 32 kDa). It encodes a novel protein that is bound to scs' but not scs in vivo. Immunostaining localizes BEAF to hundreds of interbands and many puff boundaries on… CONTINUE READING
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