Visualization of chromatin domains created by the gypsy insulator of Drosophila

@article{Byrd2003VisualizationOC,
  title={Visualization of chromatin domains created by the gypsy insulator of Drosophila},
  author={Keith Byrd and Victor G Corces},
  journal={The Journal of Cell Biology},
  year={2003},
  volume={162},
  pages={565 - 574}
}
Insulators might regulate gene expression by establishing and maintaining the organization of the chromatin fiber within the nucleus. Biochemical fractionation and in situ high salt extraction of lysed cells show that two known protein components of the gypsy insulator are present in the nuclear matrix. Using FISH with DNA probes located between two endogenous Su(Hw) binding sites, we show that the intervening DNA is arranged in a loop, with the two insulators located at the base. Mutations in… CONTINUE READING

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