Visualization of cellular focal contacts using a monoclonal antibody to 80 kD serum protein adsorbed on the substratum.

@article{Neyfakh1983VisualizationOC,
  title={Visualization of cellular focal contacts using a monoclonal antibody to 80 kD serum protein adsorbed on the substratum.},
  author={Alexander A. Neyfakh and Irina S. Tint and Tatyana M Svitkina and Alexander D Bershadsky and Vladimir I Gelfand},
  journal={Experimental cell research},
  year={1983},
  volume={149 2},
  pages={387-96}
}
We have obtained a monoclonal antibody to 80 kD protein of calf serum; this protein easily and uniformly adsorbs on glass from serum-containing media. Indirect immunofluorescence staining of chick and mouse embryo fibroblasts cultured in the presence of calf serum, fixed with formaldehyde and permeabilized with Triton X-100, revealed black non-fluorescent strips and dots under the ventral cell surface, whereas all other parts of the substratum under and between cells were highly fluorescent… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 11 extracted citations

Similar Papers

Loading similar papers…